Composite

Part:BBa_K3258030:Design

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-21)


MDV + Qbeta + CAM Resistance Gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2786
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 146
    Illegal NotI site found at 2768
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
    Illegal BglII site found at 2783
    Illegal BamHI site found at 1538
    Illegal BamHI site found at 1681
    Illegal XhoI site found at 168
    Illegal XhoI site found at 1212
    Illegal XhoI site found at 2777
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2786
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 180
    Illegal XbaI site found at 2786
    Illegal AgeI site found at 1694
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed to be used in vivo E. coli, and demonstrate evolutionary as well as replicative functionality of Qbeta and CAM resistance gene with MDV flank regions.


Source

Synthetic construct, created and designed from individual components, with CAM resistance gene and Qbeta from E. coli and bacteriophage components.

References